Ion exchange resins is one of the most widely used methods for protein separation and purification. Different proteins have different isoelectric points, different molecular sizes, different charge density distributions in the same mobile phase, different charge amounts, different binding strengths to ion exchange resin with opposite charges, and different retention times when eluting in the mobile phase, thus allowing separation.

VDO supplies 6 types of ligands, which are DEAE, CM, Q, SP, MMC, MMA. Focurose BB, Focurose FF, Focurose HP, Focurose XL, Focurose HF, Focurose HPR and Focurose HPL, which are a combination of 7 substrates and a variety of ion exchange resins to precisely match the downstream technology of bioprocesses.

Resin selection principles

★ Select ion exchange resin with fast flow rate and high loading capacity such as resin of XL matrix in the initial capture stage.

★ Moderate purification stage select ion exchange resin with high loading capacity and high resolution such as FF/XL matrix resin.

★ Fine purification stage select ion exchange resin with high resolution and high recovery rate such as HP/FF/HPR matrix resin.

★ Ion exchange resin with large particle size such as BB matrix are selected for viscous samples.

★ Select MMC or MMA resin if the sample is unstable in low salt or the sample contains polymers and monomers inside.