Size exclusion resins are based on the size of the target molecules, from the largest to the smallest peak in order to achieve the purpose of separation.
★ Size exclusion resins are often used in the subsequent purification stage with less impurities.
★ Size exclusion resins are used for the purification of smaller samples.
★ In group separation (such as desalination) can also be used in the coarse purification stage.
★ The addition of 150 mM NaCl to the buffer can effectively reduce the non-specific adsorption of the target protein.
Size exclusion resins graded separation range (globulin Da)